Human CEACAM1 ELISA Kit

Sensitivity

0.12 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat No : KE00301

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Synonyms

BGP, BGP 1, BGP1, BGPI, Biliary glycoprotein 1, CD66a, CEACAM1



Product Information

KE00301 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CEACAM1 ELISA kit is to be used to detect and quantify protein levels of endogenous CEACAM1. The assay recognizes human CEACAM1. An antibody specific for CEACAM1 has been pre-coated onto the microwells. The CEACAM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CEACAM1 is added to detect the captured CEACAM1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human CEACAM1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant, Cell lysate
Assay type Sandwich
Sensitivity 0.12 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 634

Recovery

Sample TypeAverageRange
Human serum99%86%-112%
Cell culture supernatant90%85%-97%
cell lysate102%85%-116%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12047.11.32.7
220201.76.33.1
320906.748.05.3

InterAssay

Samplenmean ( pg/mL)SDCV%
12450.13.06.0
224192.110.95.7
324822.964.37.8

Background Information

CEACAM1, also known as BGP1 or CD66a, belongs to the CEA-related cell-adhesion molecule (CEACAM) subfamily, which is a part of the immunoglobulin superfamily. It plays a role in cell-cell adhesion, regulation of cell proliferation, insulin resistance, obesity, and vascular homeostasis. CEACAM1 is expressed by certain epithelial, endothelial, lymphoid, and myeloid cells. It is found on the surface of various tumors and is associated with tumor progression, metastasis and poor prognosis. CEACAM1 serves as a specific biomarker for Non-small cell lung cancer (NSCLC) and can be used for diagnosis and prognosis of melanoma.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms BGP, BGP 1, BGP1, BGPI, Biliary glycoprotein 1, CD66a, CEACAM1