Speedy™ Human APOE One-Step ELISA Kit
Validation Data Gallery
Product Information
SE50006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOE ELISA kit is to be used to detect and quantify protein levels of endogenous APOE. The assay recognizes human APOE. An antibody specific for APOE has been pre-coated onto the microwells. Add samples and standards to the well. At the same time, another horseradish peroxidase (HRP)-conjugated antibody specific for APOE is added to detect the captured APOE protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Speedy™ Human APOE One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma,Cell culture supernatant, Urine, Human Milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL, 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 348 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 103% | 82%-126% |
| Cell culture supernatants | 105% | 81%-126% |
| Urine | 95% | 80%-113% |
| Human milk | 104% | 89%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.66 | 0.18 | 1.88 |
| 2 | 20 | 1.73 | 0.04 | 2.38 |
| 3 | 20 | 0.41 | 0.02 | 3.97 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.38 | 0.19 | 2.08 |
| 2 | 24 | 1.76 | 0.04 | 2.15 |
| 3 | 24 | 0.46 | 0.01 | 2.15 |
Background Information
APOE (apolipoprotein E) is a plasma protein implicated in lipid metabolism. It is produced predominantly by hepatocytes, macrophages, and neural cells. In the CNS, APOE is the major extracellular lipid carrier and plays a key role in neuronal protection and repair after injury. APOE is polymorphic, with three major alleles: APOE2 (cys112, cys158), APOE3 (cys112, arg158), and APOE4 (arg112, arg158). APOE2 plays a protective role against both Alzheimer's (AD) and heart disease. APOE4 confers a higher risk for atherosclerosis and Alzheimer's disease. Analysis of cerebrospinal fluid (CSF) and plasma APOE protein levels in AD patients had been performed to examine its association with AD pathology. In old age, high plasma APOE levels precede an increase of circulating CRP and strongly associates with cardiovascular mortality.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Apo E, Apo-E, apoe4, apolipoprotein E, ApolipoproteinE |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Biomater Sci Eng Liposomal-Cannabidiol Nanoformulation to Suppress HIV Replication and Reduce Oxidative Stress in Infected Microglia. | ||
J Mol Neurosci Multilayer Validation Reveals a Glia-Associated Secretome Signature in Temporal Lobe Epilepsy. |
