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验证数据展示

  • IF Staining of L02 using CL488-66549

    IF Staining of L02 using CL488-66549

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite® Plus 488-conjugated HuR/ELAVL1 antibody (CL488-66549, Clone: 1A11H2 ) at dilution of 1:100, CL594-Phalloidin (red).

  • IF Staining of HepG2 using CL488-66549

    IF Staining of HepG2 using CL488-66549

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HuR antibody (CL488-66549, Clone: 1A11H2 ) at dilution of 1:800.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated HuR/ELAVL1 Monoclonal antibody

HuR/ELAVL1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66549

产品说明书

CloneNo. 1A11H2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inL02 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66549-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66549 targets HuR/ELAVL1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag2249

Product name: Recombinant human HuR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-326 aa of BC003376

Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
别名 ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG
计算分子量 326 aa, 36 kDa
观测分子量 37 kDa
GenBank蛋白编号BC003376
基因名称 HuR/ELAVL1
Gene ID (NCBI) 1994
RRIDAB_2883354
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15717
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HuR/ELAVL1 antibody CL488-66549Download protocol
Standard Protocols
Click here to view our Standard Protocols
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