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验证数据展示

  • WB analysis using 81984-2-RR

    WB analysis using 81984-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81984-2-RR (Histone H3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 81984-2-RR

    IHC staining of mouse brain using 81984-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81984-2-RR (Histone H3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IHC staining of mouse brain using 81984-2-RR

    IHC staining of mouse brain using 81984-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81984-2-RR (Histone H3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IF Staining of HeLa using 81984-2-RR

    IF Staining of HeLa using 81984-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H3 antibody (81984-2-RR, Clone: 241079B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 81984-2-RR

    FC experiment of HepG2 using 81984-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody (81984-2-RR, Clone:241079B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 81984-2-RR

    Affinity and Kinetic Characterization of 81984-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81984-2-RR against Human Histone H3 were performed. The affinity constant is 12.2 nM.

Histone H3 Recombinant antibody

Histone H3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 81984-2-RR

产品说明书

CloneNo. 241079B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

HIST2H3A, 241079B2, H3C13, H3C14, H3C15

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, mouse brain tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Histone-H3 Ready-To-Use IHC Kit. Histone-H3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81984-2-RR targets Histone H3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Histone H3 fusion protein Ag10644 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H3a
别名 HIST2H3A, 241079B2, H3C13, H3C14, H3C15
计算分子量 136 aa, 15 kDa
观测分子量15-17 kDa
GenBank蛋白编号BC015544
基因名称 Histone H3
Gene ID (NCBI) 333932
RRIDAB_3670510
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ71DI3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

实验方案

Product Specific Protocols
WB protocol for Histone H3 antibody 81984-2-RRDownload protocol
IHC protocol for Histone H3 antibody 81984-2-RRDownload protocol
IF protocol for Histone H3 antibody 81984-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols