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验证数据展示

  • WB analysis using 18064-1-AP (same clone as 18064-1-PBS)

    WB analysis using 18064-1-AP (same clone as 18064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18064-1-AP (Histone H3.4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

    IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Histone H3.4 antibody included
  • IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

    IHC staining of mouse testis using 18064-1-AP (same clone as 18064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Histone H3.4 antibody included
  • IHC staining of rat testis using 18064-1-AP (same clone as 18064-1-PBS)

    IHC staining of rat testis using 18064-1-AP (same clone as 18064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18064-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Histone H3.4 antibody included

Histone H3.4 Polyclonal antibody, PBS Only

Histone H3.4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18064-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HIST3H3, H3.4, H3-4, H3FT, H3t

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Histone H3.4 Ready-To-Use IHC Kit. Histone H3.4 primary antibody included.

产品信息

18064-1-PBS targets Histone H3.4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12671

Product name: Recombinant human HIST3H3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-136 aa of BC101837

Sequence: MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLMREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCVIHAKRVTIMPKDIQLARRIRGERA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 3, H3
别名 HIST3H3, H3.4, H3-4, H3FT, H3t
计算分子量 136 aa, 16 kDa
观测分子量16 kDa
GenBank蛋白编号BC101837
基因名称 Histone H3.4
Gene ID (NCBI) 8290
RRIDAB_3669290
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16695
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).

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