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验证数据展示

  • WB analysis using 18064-1-AP

    WB analysis using 18064-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18064-1-AP (Histone H3.4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 18064-1-AP

    IHC staining of mouse testis using 18064-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone H3.4 antibody included
  • IHC staining of mouse testis using 18064-1-AP

    IHC staining of mouse testis using 18064-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone H3.4 antibody included
  • IHC staining of rat testis using 18064-1-AP

    IHC staining of rat testis using 18064-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18064-1-AP (Histone H3.4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone H3.4 antibody included

Histone H3.4 Polyclonal antibody

Histone H3.4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18064-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HIST3H3, H3.4, H3-4, H3FT, H3t

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, mouse testis tissue
Positive IHC detected inrat testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Histone H3.4 Ready-To-Use IHC Kit. Histone H3.4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18064-1-AP targets Histone H3.4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Histone H3.4 fusion protein Ag12671 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 3, H3
别名 HIST3H3, H3.4, H3-4, H3FT, H3t
计算分子量 136 aa, 16 kDa
观测分子量16 kDa
GenBank蛋白编号BC101837
基因名称 Histone H3.4
Gene ID (NCBI) 8290
RRIDAB_3669290
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).

实验方案

Product Specific Protocols
WB protocol for Histone H3.4 antibody 18064-1-APDownload protocol
IHC protocol for Histone H3.4 antibody 18064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols