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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68393-1-Ig (Histone H2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    FC experiment of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H2B (68393-1-Ig, Clone:1E12G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

Histone H2B Monoclonal antibody, PBS Only

Histone H2B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68393-1-PBS

产品说明书

CloneNo. 1E12G3

宿主/亚型

Mouse / IgG1

种属反应性

zebrafish, chicken, rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68393-1-PBS targets Histone H2B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with zebrafish, chicken, rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 zebrafish, chicken, rat, mouse, human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histone cluster 2, H2be
别名 HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B
计算分子量 14 kDa
观测分子量 14-17 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16778
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).