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验证数据展示

  • IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

    IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-6-RR (Hif-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

    IHC staining of mouse kidney using 82989-6-RR (same clone as 82989-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82989-6-RR (Hif-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 82989-6-RR (same clone as 82989-6-PBS)

    IF Staining of NIH/3T3 using 82989-6-RR (same clone as 82989-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Cobalt Chloride treated NIH/3T3 cells using Hif-1 alpha antibody (82989-6-RR, Clone: 230101B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82989-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82989-6-RR

    Affinity and Kinetic Characterization of 82989-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82989-6-RR against Mouse Hif1a were performed. The affinity constant is 0.163 nM.

Mouse Hif-1 alpha Recombinant monoclonal antibody, PBS Only

Hif-1 alpha Uni-rAb® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 82989-6-PBS

产品说明书

CloneNo. 230101B3

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

Hif1a, ARNT-interacting protein, Hif-1a, HIF1alpha, HIF-1-alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82989-6-PBS targets Hif-1 alpha in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag33620

Product name: Recombinant mouse Hif1a protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 530-825 aa of NM_001313919

Sequence: SEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQG*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit
别名 Hif1a, ARNT-interacting protein, Hif-1a, HIF1alpha, HIF-1-alpha
计算分子量93 kDa
GenBank蛋白编号NM_001313919
基因名称 Hif1a
Gene ID (NCBI) 15251
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ61221
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia

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