Hexokinase 2 Recombinant antibody

Hexokinase 2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85875-1-RR

产品说明书

CloneNo. 250190C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HCT 116 cells, mouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85875-1-RR targets Hexokinase 2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Hexokinase 2 fusion protein Ag16895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexokinase 2
别名 HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2
计算分子量 102 kDa
观测分子量 102-120 kDa
GenBank蛋白编号BC021116
基因名称 Hexokinase 2
Gene ID (NCBI) 3099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52789
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).

实验方案

Product Specific Protocols
WB protocol for Hexokinase 2 antibody 85875-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...