验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HCT 116 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85875-1-RR targets Hexokinase 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Hexokinase 2 fusion protein Ag16895 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | hexokinase 2 |
| 别名 | HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2 |
| 计算分子量 | 102 kDa |
| 观测分子量 | 102-120 kDa |
| GenBank蛋白编号 | BC021116 |
| 基因名称 | Hexokinase 2 |
| Gene ID (NCBI) | 3099 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P52789 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Hexokinase 2 antibody 85875-1-RR | Download protocol |
| IHC protocol for Hexokinase 2 antibody 85875-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
