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验证数据展示

  • WB analysis using 85875-1-RR (same clone as 85875-1-PBS)

    WB analysis using 85875-1-RR (same clone as 85875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02173-3

    Sandwich ELISA standard curve of MP02173-3

    Sandwich ELISA standard curve of MP02173-3, Human Hexokinase 2 Recombinant Matched Antibody Pair - PBS only. 85875-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16895. 85875-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP02173-3

    Sample test of MP02173-3

    The mean Hexokinase 2 concentration was determined to be 321.70 ng/mL in A549 cell extract based on a 1.3 mg/mL extract load.

  • Sample test of MP02173-3

    Sample test of MP02173-3

    Serum of sixteen individual healthy human donors was measured. The human Hexokinase 2 concentration of detected samples was determined to be 1.16 ng/mL with a range of 0.35 - 4.27 ng/mL.

  • IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

    IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HK2 antibody included
  • IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

    IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HK2 antibody included
  • Affinity and Kinetic Characterization of 85875-1-RR

    Affinity and Kinetic Characterization of 85875-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85875-1-RR against Human Hexokinase 2 were performed. The affinity constant is 0.302 nM.

Hexokinase 2 Recombinant antibody, PBS Only (Detector)

Hexokinase 2 Uni-rAbTM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85875-1-PBS

产品说明书

CloneNo. 250190C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

别名

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.

产品信息

85875-1-PBS targets Hexokinase 2 as part of a matched antibody pair:

MP02173-3: 85875-3-PBS capture and 85875-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 Hexokinase 2 fusion protein Ag16895 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexokinase 2
别名 HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2
计算分子量 102 kDa
观测分子量 102-120 kDa
GenBank蛋白编号BC021116
基因名称 Hexokinase 2
Gene ID (NCBI) 3099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52789
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).