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验证数据展示

  • WB analysis of human plasma using 66229-1-Ig (same clone as 66229-1-PBS)

    WB analysis of human plasma using 66229-1-Ig (same clone as 66229-1-PBS)

    0.5 μl human plasma was subjected to SDS PAGE followed by western blot with 66229-1-Ig (HP Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HP antibody included
  • IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    IHC staining of human liver cancer using 66229-1-Ig (same clone as 66229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66229-1-Ig (Haptoglobin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HP antibody included

Haptoglobin Monoclonal antibody, PBS Only

Haptoglobin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66229-1-PBS

产品说明书

CloneNo. 3B4B9

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, Indirect ELISA

别名

BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HP Ready-To-Use IHC Kit. HP primary antibody included.

产品信息

66229-1-PBS targets Haptoglobin in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag9927

Product name: Recombinant human HP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 179-406 aa of BC058031

Sequence: MVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 haptoglobin
别名 BP, HAPT, haptoglobin, HP, HP2 ALPHA 2, HPA1S, Zonulin
计算分子量281aa,31 kDa; 228aa,25 kDa
观测分子量 42 kDa
GenBank蛋白编号BC058031
基因名称 HP
Gene ID (NCBI) 3240
RRIDAB_2881620
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00738
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn’s disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).