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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98362-2-RR

    FC experiment of human peripheral blood leukocytes using 98362-2-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98362-2-RR

    FC experiment of human peripheral blood leukocytes using 98362-2-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HVEM/TNFRSF14 Rabbit RecAb (98362-2-RR, Clone: 250728C11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98362-2-RR

    Affinity and Kinetic Characterization of 98362-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98362-2-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 2.22 nM.

Anti-Human HVEM/TNFRSF14 Rabbit Recombinant Antibody

HVEM/TNFRSF14 Uni-rAb® Recombinant Antibody for FC
Cat No. 98362-2-RR

产品说明书

CloneNo. 250728C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98362-2-RR targets HVEM/TNFRSF14 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1275

Product name: Recombinant Human HVEM/TNFRSF14 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 39-202 aa of BC002794

Sequence: LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
别名 TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A
计算分子量 30 kDa
GenBank蛋白编号BC002794
基因名称 TNFRSF14
Gene ID (NCBI) 8764
RRIDAB_3746012
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92956
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.

实验方案

Product Specific Protocols
FC protocol for HVEM/TNFRSF14 antibody 98362-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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