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验证数据展示

  • IF Staining of HepG2 using CL488-68053

    IF Staining of HepG2 using CL488-68053

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HTRA1 antibody (CL488-68053, Clone: 1B11G12 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-68053

    FC experiment of HeLa using CL488-68053

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Htra1 Monoclonal Antibody (CL488-68053, Clone:1B11G12)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated HTRA1 Monoclonal antibody

HTRA1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68053

产品说明书

CloneNo. 1B11G12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC, FC (Intra)

别名

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68053-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68053 targets HTRA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 HTRA1 fusion protein Ag31204 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 HtrA serine peptidase 1
别名 PRSS11, HtrA, EC:3.4.21.-, 1B11G12
计算分子量 51 kDa
观测分子量 51 kDa
GenBank蛋白编号NM_002775
基因名称 HTRA1
Gene ID (NCBI) 5654
RRIDAB_3672969
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92743
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HTRA1 antibody CL488-68053Download protocol
FC protocol for CL Plus 488 HTRA1 antibody CL488-68053Download protocol
Standard Protocols
Click here to view our Standard Protocols