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验证数据展示

  • WB analysis using 86275-1-RR (same clone as 86275-1-PBS)

    WB analysis using 86275-1-RR (same clone as 86275-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86275-1-RR (HSP60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    WB analysis of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    WB result of HSP60 antibody (86275-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP60 transfected HeLa cells. This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    IF Staining of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP60 antibody (86275-1-RR, Clone: 250248D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86275-1-RR

    Affinity and Kinetic Characterization of 86275-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86275-1-RR against Human HSPD1 were performed. The affinity constant is 7.43 nM.

  • Featured Product
  • KD/KO验证

HSP60 Recombinant monoclonal antibody, PBS Only

HSP60 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86275-1-PBS

产品说明书

CloneNo. 250248D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

HSPD1, 60 kDa chaperonin, Chaperonin 60, CPN60, EC:5.6.1.7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.

产品信息

86275-1-PBS targets HSP60 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7401

Product name: Recombinant human HSPD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-573 aa of BC003030

Sequence: SPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock 60kDa protein 1 (chaperonin)
别名 HSPD1, 60 kDa chaperonin, Chaperonin 60, CPN60, EC:5.6.1.7
计算分子量 61 kDa
观测分子量 60 kDa
GenBank蛋白编号BC003030
基因名称 HSP60
Gene ID (NCBI) 3329
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10809
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

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