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验证数据展示

  • WB analysis using 32468-1-AP (same clone as 32468-1-PBS)

    WB analysis using 32468-1-AP (same clone as 32468-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32468-1-AP (HSPA4L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 32468-1-AP (same clone as 32468-1-PBS)

    IHC staining of human lung cancer using 32468-1-AP (same clone as 32468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 32468-1-AP (same clone as 32468-1-PBS)

    IHC staining of human tonsillitis using 32468-1-AP (same clone as 32468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

HSPA4L Polyclonal antibody, PBS Only

HSPA4L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32468-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

APG1, Heat shock 70 kDa protein 4L, Heat shock 70-related protein APG-1, Heat shock protein family H member 3, Heat-shock protein family A member 4-like protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32468-1-PBS targets HSPA4L in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38354

Product name: Recombinant human HSPA4L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 690-839 aa of NM_001317381.1

Sequence: MKYMEHEERPKALNDLGKKIQLVMKVIEAYRNKDERYDHLDPTEMEKVEKCISDAMSWLNSKMNAQNKLSLTQDPVVKVSEIVAKSKELDNFCNPIIYKPKPKAEVPEDKPKANSEHNGPMDGQSGTETKSDSTKDSSQHTKSSGEMEVD*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 70kDa protein 4-like
别名 APG1, Heat shock 70 kDa protein 4L, Heat shock 70-related protein APG-1, Heat shock protein family H member 3, Heat-shock protein family A member 4-like protein
计算分子量95kDa，839aa
观测分子量95~100 kDa
GenBank蛋白编号NM_001317381.1
基因名称 HSPA4L
Gene ID (NCBI) 22824
RRIDAB_3742620
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95757
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPA4L (Heat shock 70-kDa protein 4-like), also known as APG1 or OSP94, is a member of the heat shock protein 110 family, whose mRNA is strongly expressed in normal human testis and overexpressed in leukemia cells. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells. Up-regulation of the Hspa4l gene in kidney and renal cell lines by osmotic stress suggested that Hspa4l may function to enable the kidney to compensate for osmotic stress present within the kidney (PMID: 16923965, 17588478).

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