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  • Featured Product
  • KD/KO Validated

HSP90AB1 Recombinant antibody

HSP90AB1 Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5P14

Cat No : 80301-1-RR

Print datasheet

Synonyms

D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB

验证数据展示

  • IHC staining of human breast cancer using 80301-1-RR

    IHC staining of human breast cancer using 80301-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AB1 antibody included
  • IF Staining of HeLa using 80301-1-RR

    IF Staining of HeLa using 80301-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 80301-1-RR

    WB analysis using 80301-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 80301-1-RR

    WB analysis of HEK-293 using 80301-1-RR

    WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inLNCaP cells, zebrafish tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80301-1-RR targets HSP90AB1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, zebrafish
Immunogen HSP90AB1 fusion protein Ag1947 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Synonyms D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB
Calculated Molecular Weight 723 aa, 83 kDa
GenBank Accession NumberBC014485
Gene Symbol HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2918885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.

实验方案

Product Specific Protocols
WB protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
IHC protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
IF protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols