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验证数据展示

  • IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

    IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP90AB1 antibody included
  • IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

    IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

    WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

    WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

    WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80301-1-RR

    Affinity and Kinetic Characterization of 80301-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80301-1-RR against Human HSP90AB1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

HSP90 Recombinant monoclonal antibody, PBS Only

HSP90 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80301-1-PBS

产品说明书

CloneNo. 5P14

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HSP90AB1, 5P14, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.

产品信息

80301-1-PBS targets HSP90 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原

CatNo: Ag1947

Product name: Recombinant human HSP90AB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-710 aa of BC014485

Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock protein 90kDa alpha (cytosolic), class B member 1
别名 HSP90AB1, 5P14, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta
计算分子量 723 aa, 83 kDa
观测分子量 83-90 kDa
GenBank蛋白编号BC014485
基因名称 HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2918885
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08238
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

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