CoraLite®594-conjugated HSP60 Monoclonal antibody
HSP60 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2F10E7
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66041-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 1 publications below |
产品信息
The immunogen of CL594-66041 is HSP60 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC |
| Cited Applications | FC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | HSP60 fusion protein Ag7848 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Synonyms | 60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, Heat shock protein 60, HLD4, HSP 60, HSP60, HSP65, HSPD1, HuCHA60, Mitochondrion Marker, P60 lymphocyte protein, SPG13 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC003030 |
| Gene Symbol | HSPD1 |
| Gene ID (NCBI) | 3329 |
| RRID | AB_2883487 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P10809 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HSP60 antibody CL594-66041 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis |