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CoraLite®594-conjugated HSP47 Monoclonal antibody

HSP47 Monoclonal Antibody for IF/ICC, FC (intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, FC (intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A8F6

Cat No : CL594-67863

Print datasheet

Synonyms

47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2

验证数据展示

  • IF Staining of HeLa using CL594-67863

    IF Staining of HeLa using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-67863

    IF Staining of HepG2 using CL594-67863

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 HSP47 antibody (CL594-67863, Clone: 2A8F6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-67863

    FC experiment of HepG2 using CL594-67863

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human HSP47 (CL594-67863, Clone:2A8F6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67863-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67863 targets HSP47 in IF, FC (intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, FC (intra)
Tested Reactivity Human, mouse, rat
Immunogen HSP47 fusion protein Ag30999 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Synonyms 47 kDa heat shock protein, AsTP3, CBP1, CBP2, Collagen binding protein, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC014623
Gene Symbol SERPINH1
Gene ID (NCBI) 871
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50454
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 HSP47 antibody CL594-67863Download protocol
Standard Protocols
Click here to view our Standard Protocols