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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of human skeletal muscle using 21755-1-AP

    WB analysis of human skeletal muscle using 21755-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

  • IHC staining of human heart using 21755-1-AP

    IHC staining of human heart using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 21755-1-AP

    IHC staining of human heart using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21755-1-AP

    IF Staining of HepG2 using 21755-1-AP

    Immunofluorescent analysis of HepG2 cells, using HSPB2 antibody 21755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

HSPB2 Polyclonal antibody

HSPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21755-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Heat shock protein family B member 2, Heat shock protein beta-2, Heat shock protein beta 2, heat shock 27kDa protein 2, DMPK-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21755-1-AP targets HSPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, rat
文献引用反应性human
免疫原 HSPB2 fusion protein Ag16146 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 27kDa protein 2
别名 Heat shock protein family B member 2, Heat shock protein beta-2, Heat shock protein beta 2, heat shock 27kDa protein 2, DMPK-binding protein
计算分子量 182 aa, 20 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC109393
基因名称 HSPB2
Gene ID (NCBI) 3316
RRIDAB_10859789
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16082
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSPB2 antibody 21755-1-APDownload protocol
IHC protocol for HSPB2 antibody 21755-1-APDownload protocol
IF protocol for HSPB2 antibody 21755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cancer

miR-17-5p promotes the invasion and migration of colorectal cancer by regulating HSPB2.

Authors - Weifang Yu
View Article
humanWB,IHC

Clin Epigenetics

PMID: 31088577

Authors - An-An Yin
View Article