验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
21755-1-AP targets HSPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag16146 Product name: Recombinant human HSP27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC109393 Sequence: MSGRSVPHAHPATAEYEFANPSRLGEQRFGEGLLPEEILTPTLYHGYYVRPRAAPAGEGSRAGASELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYVLPADVDPWRVRAALSHDGILNLEAPRGGRHLDTEVNEVYISLLPAPPDPEEEEEAAIVEP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock 27kDa protein 2 |
| 别名 | Heat shock protein family B member 2, Heat shock protein beta-2, Heat shock protein beta 2, heat shock 27kDa protein 2, DMPK-binding protein |
| 计算分子量 | 182 aa, 20 kDa |
| 观测分子量 | 20-25 kDa |
| GenBank蛋白编号 | BC109393 |
| 基因名称 | HSPB2 |
| Gene ID (NCBI) | 3316 |
| RRID | AB_10859789 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16082 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HSPB2 antibody 21755-1-AP | Download protocol |
| IHC protocol for HSPB2 antibody 21755-1-AP | Download protocol |
| WB protocol for HSPB2 antibody 21755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cancer miR-17-5p promotes the invasion and migration of colorectal cancer by regulating HSPB2. | ||
Front Immunol Transcriptional profiling reveals H.pylori-associated genes induced inflammatory cell infiltration and chemoresistance in gastric cancer
| ||
