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HSPB2 Polyclonal antibody

HSPB2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21755-1-AP

Print datasheet

Synonyms

heat shock 27kDa protein 2, HSPB2

验证数据展示

  • WB analysis of human skeletal muscle using 21755-1-AP

    WB analysis of human skeletal muscle using 21755-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

  • IHC staining of human heart using 21755-1-AP

    IHC staining of human heart using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 21755-1-AP

    IHC staining of human heart using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21755-1-AP

    IF Staining of HepG2 using 21755-1-AP

    Immunofluorescent analysis of HepG2 cells, using HSPB2 antibody 21755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21755-1-AP is HSPB2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen HSPB2 fusion protein Ag16146 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 27kDa protein 2
Synonyms heat shock 27kDa protein 2, HSPB2
Calculated Molecular Weight 182 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC109393
Gene Symbol HSPB2
Gene ID (NCBI) 3316
RRIDAB_10859789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16082
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSPB2 antibody 21755-1-APDownload protocol
IHC protocol for HSPB2 antibody 21755-1-APDownload protocol
IF protocol for HSPB2 antibody 21755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cancer

miR-17-5p promotes the invasion and migration of colorectal cancer by regulating HSPB2.

Authors - Weifang Yu
View Article
humanWB,IHC

Clin Epigenetics

PMID: 31088577

Authors - An-An Yin
View Article