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验证数据展示

  • WB analysis of Jurkat using 17765-1-AP (same clone as 17765-1-PBS)

    WB analysis of Jurkat using 17765-1-AP (same clone as 17765-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17765-1-AP (HSF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17765-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 17765-1-AP (same clone as 17765-1-PBS)

    IF Staining of mouse testis using 17765-1-AP (same clone as 17765-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using HSF5 antibody (17765-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17765-1-PBS in a different storage buffer formulation.

HSF5 Polyclonal antibody, PBS Only

HSF5 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 17765-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, Indirect ELISA

别名

HSTF5, HSTF 5, HSF 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17765-1-PBS targets HSF5 in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12168

Product name: Recombinant human HSF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033017

Sequence: MEALLSTPINPNNFPAKLWRLVNSPRYRSIRWDGRGEGLLIDQPLFEAELLSPPGPGGGGGTAGAGAEPELFKTTSFTSFIRQLNLYGFRKVVLGGPGGGKPAGNGPLHHFHNPHFRRDQPQLLVHLKRLTSANKAKLAAGLEVPCRPPNRFQRLLITSASAATAPLQHQQPPPPAGPRPEPHGPVAVGQFHRSFRRDSLSPYSCVSTPSHDHSTYPLKGLDRTPVPHRIWQNSLGMHPGQVETSPTFSDKGVPFPVLQRFPTEVTYTLQPSTTSVHVQQGPQTMVSSSQKYSNYTPSAQYSQAYYPTAVLQCCSPTHMDALSSCVTPTASSYAHCNYFQNPSMQSSYPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor family member 5
别名 HSTF5, HSTF 5, HSF 5
计算分子量 596 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC033017
基因名称 HSF5
Gene ID (NCBI) 124535
RRIDAB_2233301
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4G112
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heat shock transcription factor 5 (HSF5) is a member of the HSF family, which are well-known transcriptional regulators of genes encoding heat shock proteins and other types of proteins and have functions in reproduction, the immune response, and the aging process. HSF5 is a non-canonical HSF that possesses a winged- helix-turn-helix (WHTH)-like DNA-binding domain and is specifically expressed in the testis (PMID: 38958533, PMID: 39162221).

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