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验证数据展示

  • WB analysis of Jurkat using 17765-1-AP

    WB analysis of Jurkat using 17765-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17765-1-AP (HSF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 17765-1-AP

    IF Staining of mouse testis using 17765-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using HSF5 antibody (17765-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSF5 Polyclonal antibody

HSF5 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 17765-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, ELISA

别名

HSTF5, HSTF 5, HSF 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17765-1-AP targets HSF5 in WB, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12168

Product name: Recombinant human HSF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033017

Sequence: MEALLSTPINPNNFPAKLWRLVNSPRYRSIRWDGRGEGLLIDQPLFEAELLSPPGPGGGGGTAGAGAEPELFKTTSFTSFIRQLNLYGFRKVVLGGPGGGKPAGNGPLHHFHNPHFRRDQPQLLVHLKRLTSANKAKLAAGLEVPCRPPNRFQRLLITSASAATAPLQHQQPPPPAGPRPEPHGPVAVGQFHRSFRRDSLSPYSCVSTPSHDHSTYPLKGLDRTPVPHRIWQNSLGMHPGQVETSPTFSDKGVPFPVLQRFPTEVTYTLQPSTTSVHVQQGPQTMVSSSQKYSNYTPSAQYSQAYYPTAVLQCCSPTHMDALSSCVTPTASSYAHCNYFQNPSMQSSYPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor family member 5
别名 HSTF5, HSTF 5, HSF 5
计算分子量 596 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC033017
基因名称 HSF5
Gene ID (NCBI) 124535
RRIDAB_2233301
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4G112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heat shock transcription factor 5 (HSF5) is a member of the HSF family, which are well-known transcriptional regulators of genes encoding heat shock proteins and other types of proteins and have functions in reproduction, the immune response, and the aging process. HSF5 is a non-canonical HSF that possesses a winged- helix-turn-helix (WHTH)-like DNA-binding domain and is specifically expressed in the testis (PMID: 38958533, PMID: 39162221).

实验方案

Product Specific Protocols
IF protocol for HSF5 antibody 17765-1-APDownload protocol
WB protocol for HSF5 antibody 17765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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