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验证数据展示

  • WB analysis using 67496-1-Ig

    WB analysis using 67496-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67496-1-Ig (HSD17B6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67496-1-Ig

    IHC staining of human liver cancer using 67496-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67496-1-Ig (HSD17B6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67496-1-Ig

    IHC staining of human liver cancer using 67496-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67496-1-Ig (HSD17B6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSD17B6 Monoclonal antibody

HSD17B6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67496-1-Ig

产品说明书

CloneNo. 2D4G6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJAR cells, Daudi cells, HuH-7 cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67496-1-Ig targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag17330

Product name: Recombinant human HSD17B6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-317 aa of BC020710

Sequence: MWLYLAAFVGLYYLLHWYRERQVVSHLQDKYVFITGCDSGFGNLLARQLDARGLRVLAACLTEKGAEQLRGQTSDRLETVTLDVTKMESIAAATQWVKEHVGDRGLWGLVNNAGILTPITLCEWLNTEDSMNMLKVNLIGVIQVTLSMLPLVRRARGRIVNVSSILGRVAFFVGGYCVSKYGVEAFSDILRREIQHFGVKISIVEPGYFRTGMTNMTQSLERMKQSWKEAPKHIKETYGQQYFDALYNIMKEGLLNCSTNLNLVTDCMEHALTSVHPRTRYSAGWDAKFFFIPLSYLPTSLADYILTRSWPKPAQAV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
别名 17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6
计算分子量 317 aa, 36 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC020710
基因名称 HSD17B6
Gene ID (NCBI) 8630
RRIDAB_2882720
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO14756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HSD17B6 antibody 67496-1-IgDownload protocol
WB protocol for HSD17B6 antibody 67496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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