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验证数据展示

  • WB analysis using 27461-1-AP (same clone as 27461-1-PBS)

    WB analysis using 27461-1-AP (same clone as 27461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 27461-1-AP (same clone as 27461-1-PBS)

    IF Staining of HepG2 using 27461-1-AP (same clone as 27461-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSD17B12 antibody (27461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSD17B12 antibody included
  • IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSD17B12 antibody included

HSD17B12 Polyclonal antibody, PBS Only

HSD17B12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27461-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

17 beta HSD 12, 17-beta-HSD 12, 17-beta-hydroxysteroid dehydrogenase 12, 3 ketoacyl CoA reductase, 3-ketoacyl-CoA reductase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HSD17B12 Ready-To-Use IHC Kit. HSD17B12 primary antibody included.

产品信息

27461-1-PBS targets HSD17B12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26540

Product name: Recombinant human HSD17B12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-271 aa of BC012043

Sequence: SATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 12
别名 17 beta HSD 12, 17-beta-HSD 12, 17-beta-hydroxysteroid dehydrogenase 12, 3 ketoacyl CoA reductase, 3-ketoacyl-CoA reductase
计算分子量 312 aa, 34 kDa
观测分子量30 kDa
GenBank蛋白编号BC012043
基因名称 HSD17B12
Gene ID (NCBI) 51144
RRIDAB_3669604
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).

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