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验证数据展示

  • WB analysis using 27461-1-AP

    WB analysis using 27461-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27461-1-AP

    IF Staining of HepG2 using 27461-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSD17B12 antibody (27461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HSD17B12 Polyclonal antibody

HSD17B12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27461-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:1.1.1.330, 3-ketoacyl-CoA reductase, 3 ketoacyl CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HCT 116 cells, HEK-293 cells, HT-29 cells, U-251 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSD17B12 Ready-To-Use IHC Kit. HSD17B12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27461-1-AP targets HSD17B12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HSD17B12 fusion protein Ag26540 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 12
别名 EC:1.1.1.330, 3-ketoacyl-CoA reductase, 3 ketoacyl CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12
计算分子量 312 aa, 34 kDa
观测分子量30 kDa
GenBank蛋白编号BC012043
基因名称 HSD17B12
Gene ID (NCBI) 51144
RRIDAB_3669604
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).

实验方案

Product Specific Protocols
WB protocol for HSD17B12 antibody 27461-1-APDownload protocol
IF protocol for HSD17B12 antibody 27461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols