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验证数据展示

  • WB analysis of rat liver using 85185-4-RR

    WB analysis of rat liver using 85185-4-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 85185-4-RR (HSCB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85185-4-RR

    Affinity and Kinetic Characterization of 85185-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85185-4-RR against Human HSCB were performed. The affinity constant is below 1 pM.

HSCB Recombinant monoclonal antibody

HSCB Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85185-4-RR

产品说明书

CloneNo. 242836H3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

242836H3, DNAJC20, HSC20, Iron-sulfur cluster co-chaperone protein HscB, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85185-4-RR targets HSCB in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag7268

Product name: Recombinant human HSCB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of BC065569

Sequence: MWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCGGPWGPGREDRFFCPQCRALQAPDPTRDYFSLMDCNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYKTLLAPLSRGLYLLKLHGIEIPERTDYEMDRQFLIEIMEINEKLAEAESEAAMKEIESIVKAKQKEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEKIKLKKIPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HscB iron-sulfur cluster co-chaperone homolog (E. coli)
别名 242836H3, DNAJC20, HSC20, Iron-sulfur cluster co-chaperone protein HscB, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic
计算分子量 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC065569
基因名称 HSCB
Gene ID (NCBI) 150274
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSCB, also named as HSC20 and DNAJC20, acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria.

实验方案

Product Specific Protocols
WB protocol for HSCB antibody 85185-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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