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验证数据展示

  • IF Staining of human breast cancer using CL594-66490

    IF Staining of human breast cancer using CL594-66490

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66490 (HRPT2; CDC73 antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using CL594-66490

    IF Staining of MCF-7 using CL594-66490

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 HRPT2, CDC73 antibody (CL594-66490, Clone: 4B4H2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated HRPT2, CDC73 Monoclonal antibody

HRPT2, CDC73 Monoclonal Antibody for IF/ICC
Cat No. CL594-66490

产品说明书

CloneNo. 4B4H2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

CDC73, HRPT2, HRPT2; CDC73, 4B4H2, C1orf28

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDC73 Ready-To-Use IHC Kit. CDC73 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66490-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66490 targets HRPT2, CDC73 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag2959

Product name: Recombinant human HRPT2; CDC73 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-409 aa of BC014351

Sequence: WRTRTTILQSTGKNFSKNIFAILQSVKAREEGRAPEQRPAPNAAPVDPTLRTKQPIPAAYNRYDQERFKGKEETEGFKIDTMGTYHGMTLKSVTEGASARKTQTPAAQPVPRPVSQARPPPNQKKGSRTPIIIIPAATTSLITMLNAKDLLQDLKFVPSDEKKKQGCQRENETLIQRRKDQMQPGGTAISVTVPYRVVDQPLKLMPQDWDRVVAVFVQGPAWQFKGWPWLLPDGSPVDIFAKIKAFHLKYDEVRLDPNVQKWDVTVLELSYHKRHLDRPVFLRFWETLDRYMVKHKSHLRF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
别名 CDC73, HRPT2, HRPT2; CDC73, 4B4H2, C1orf28
计算分子量 531 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC014351
基因名称 HRPT2/CDC73
Gene ID (NCBI) 79577
RRIDAB_2883594
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6P1J9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 HRPT2, CDC73 antibody CL594-66490Download protocol
Standard Protocols
Click here to view our Standard Protocols
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