×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

    WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

    WB analysis of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

    WB result of HRAS-Specific antibody (18295-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRAS-Specific transfected HEK-293 cells. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

    WB analysis using 18295-1-AP (same clone as 18295-1-PBS)

    Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

    WB analysis of HEK-293 using 18295-1-AP (same clone as 18295-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18295-1-PBS

    IHC staining of human kidney using 18295-1-PBS

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 18295-1-AP (HRAS-Specific Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with HRAS antibody included
  • IP experiment of mouse brain using 18295-1-AP (same clone as 18295-1-PBS)

    IP experiment of mouse brain using 18295-1-AP (same clone as 18295-1-PBS)

    IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 18295-1-AP (same clone as 18295-1-PBS)

    IF Staining of MCF-7 using 18295-1-AP (same clone as 18295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HRAS-Specific antibody (18295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 18295-1-AP (same clone as 18295-1-PBS)

    FC experiment of HeLa using 18295-1-AP (same clone as 18295-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 18295-1-AP (same clone as 18295-1-PBS)

    IHC staining of human colon cancer using 18295-1-AP (same clone as 18295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 18295-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HRAS antibody included
  • Featured Product
  • KD/KO Validated

HRAS-Specific Polyclonal antibody, PBS Only

HRAS-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 18295-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

GTPase HRas, H RASIDX, HRAS, HRAS1, EC:3.6.5.2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HRAS Ready-To-Use IHC Kit. HRAS primary antibody included.

产品信息

18295-1-PBS targets HRAS-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-Ha-ras Harvey rat sarcoma viral oncogene homolog
别名 GTPase HRas, H RASIDX, HRAS, HRAS1, EC:3.6.5.2
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC006499
基因名称 HRAS
Gene ID (NCBI) 3265
RRIDAB_2121046
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01112
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

||
New chat

Able™

正在加载，请稍候...