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验证数据展示

  • FC experiment of HeLa using 82682-13-RR

    FC experiment of HeLa using 82682-13-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HRAS-Specific Recombinant Antibody (82682-13-RR, Clone:242062C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of HepG2 using 82682-13-RR

    FC experiment of HepG2 using 82682-13-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HRAS-Specific Recombinant Antibody (82682-13-RR, Clone:242062C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

HRAS Recombinant monoclonal antibody

HRAS Uni-rAb® Recombinant Antibody for FC (Intra), ELISA
Cat No. 82682-13-RR

产品说明书

CloneNo. 242062C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

GTPase HRas, H RASIDX, HRAS1, CTLO, c-H-ras

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HRAS Ready-To-Use IHC Kit. HRAS primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82682-13-RR targets HRAS in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-Ha-ras Harvey rat sarcoma viral oncogene homolog
别名 GTPase HRas, H RASIDX, HRAS1, CTLO, c-H-ras
计算分子量 21 kDa
GenBank蛋白编号BC006499
基因名称 HRAS
Gene ID (NCBI) 3265
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP01112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

实验方案

Product Specific Protocols
FC protocol for HRAS antibody 82682-13-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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