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CoraLite® Plus 488-conjugated HPRT1 Monoclonal antibody

HPRT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3D1

Cat No : CL488-67518

Print datasheet

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1

验证数据展示

  • IF Staining of HeLa using CL488-67518

    IF Staining of HeLa using CL488-67518

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488-conjugated HPRT1 antibody (CL488-67518, Clone: 1C3D1 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67518-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67518 targets HPRT1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen HPRT1 fusion protein Ag7044 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hypoxanthine phosphoribosyltransferase 1
Synonyms HGPRT, HGPRTase, HPRT, HPRT1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
RRIDAB_2919488
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00492
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPRT1, also named as HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. 67518-1-Ig antibody detects the protein around 28 kDa in SDS-PAGE. And HPRT1 deficient cell line Sp2/0 was served as a negative control in PTG's results.(PMID:20544509, 28045594, 27379977)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HPRT1 antibody CL488-67518Download protocol
Standard Protocols
Click here to view our Standard Protocols