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验证数据展示

  • WB analysis using 31387-1-AP

    WB analysis using 31387-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31387-1-AP (HOXB8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 31387-1-AP

    WB analysis using 31387-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31387-1-AP (HOXB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HOXB8 Polyclonal antibody

HOXB8 Polyclonal Antibody for WB, ELISA
Cat No. 31387-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Hox 2.4, HOX2D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, HCT 116 cells, HT-29 cells, LoVo cells, mouse epididymis tissue, rat kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31387-1-AP targets HOXB8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34946

Product name: Recombinant human HOXB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 15-134 aa of NM_024016

Sequence: TGESLRPNYYDCGFAQDLGGRPTVVYGPSSGGSFQHPSQIQEFYHGPSSLSTAPYQQNPCAVACHGDPGNFYGYDPLQRQSLFGAQDPDLVQYADCKLAAASGLGEEAEGSEQSPSPTQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox B8
别名 Hox 2.4, HOX2D
计算分子量28 kDa
观测分子量31 kDa
GenBank蛋白编号NM_024016
基因名称 HOXB8
Gene ID (NCBI) 3218
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP17481
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOXB8, also known as homeobox B8, is a member of the homeobox family. HOXB8 plays a role in the regulation of embryonic development. It helps determine the identity and differentiation of cells along the anterior-posterior axis of the body. ncreased expression of HOXB8 is associated with colorectal cancer. It has been found to enhance the proliferation and metastasis of colorectal cancer cells by promoting epithelial-mesenchymal transition (EMT) via STAT3 activation (PMID: 30622439).

实验方案

Product Specific Protocols
WB protocol for HOXB8 antibody 31387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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