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验证数据展示

  • IF Staining of HepG2 using CL488-83155-4

    IF Staining of HepG2 using CL488-83155-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HOXB6 antibody (CL488-83155-4, Clone: 230433F10 ) at dilution of 1:200.

  • FC experiment of K-562 using CL488-83155-4

    FC experiment of K-562 using CL488-83155-4

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HOXB6 Recombinant antibody (CL488-83155-4, Clone:230433F10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated HOXB6 Recombinant monoclonal antibody

HOXB6 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83155-4

产品说明书

CloneNo. 230433F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

缓冲液配方:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 83155-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83155-4 targets HOXB6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag14189

Product name: Recombinant human HOXB6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC014651

Sequence: MSSYFVNSTFPVTLASGQESFLGQLPLYSSGYADPLRHYPAPYGPGPGQDKGFATSSYYPPAGGGYGRAAPCDYGPAPAFYREKESACALSGADEQPPFHPEPRKSDCAQDKSVFGETEEQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 homeobox B6
别名 Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2
计算分子量 224 aa, 25 kDa
GenBank蛋白编号BC014651
基因名称 HOXB6
Gene ID (NCBI) 3216
RRIDAB_3673205
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
激发激光Blue laser (488 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17509
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOXB6 is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HOXB6 antibody CL488-83155-4Download protocol
IF protocol for CL Plus 488 HOXB6 antibody CL488-83155-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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