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验证数据展示

  • WB analysis using 85692-4-RR

    WB analysis using 85692-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85692-4-RR

    WB analysis using 85692-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85692-4-RR

    IHC staining of mouse brain using 85692-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 85692-4-RR

    IHC staining of mouse brain using 85692-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85692-4-RR

    Affinity and Kinetic Characterization of 85692-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

HOMER1 Recombinant antibody

HOMER1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85692-4-RR

产品说明书

CloneNo. 243007G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, mouse brain tissue, MOLT-4 cells, HEK-293 cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85692-4-RR targets HOMER1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HOMER1 fusion protein Ag3109 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 homer homolog 1 (Drosophila)
别名 HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1
计算分子量 40 kDa
观测分子量 46-48 kDa
GenBank蛋白编号BC015502
基因名称 HOMER1
Gene ID (NCBI) 9456
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86YM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

实验方案

Product Specific Protocols
WB protocol for HOMER1 antibody 85692-4-RRDownload protocol
IHC protocol for HOMER1 antibody 85692-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols