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验证数据展示

  • WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

    WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

  • WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

    WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

    IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

    IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85692-4-RR

    Affinity and Kinetic Characterization of 85692-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

HOMER1 Recombinant antibody, PBS Only

HOMER1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85692-4-PBS

产品说明书

CloneNo. 243007G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85692-4-PBS targets HOMER1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HOMER1 fusion protein Ag3109 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 homer homolog 1 (Drosophila)
别名 HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1
计算分子量 40 kDa
观测分子量 46-48 kDa
GenBank蛋白编号BC015502
基因名称 HOMER1
Gene ID (NCBI) 9456
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86YM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.