验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, NR8383 cells, NIH/3T3 cells, RAW 264.7 cells, 4T1 cells, mouse liver tissue, mouse kidney tissue, mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83291-3-RR targets HO-1/Hmox1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg0938 Product name: Recombinant mouse HO-1/Hmox1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 1-266 aa of NM_010442.2 Sequence: MERPQPDSMPQDLSEALKEATKEVHIQAENAEFMKNFQKGQVSREGFKLVMASLYHIYTALEEEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEIIPCTPATQHYVKRLHEVGRTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPNIDSPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQVMLTEEHKDQSPSQMASLRQRPASLVQDTAPAETPRGKPQISTSSSQT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | heme oxygenase (decycling) 1 |
| 别名 | Hmox1, HO-1, 240236G4, D8Wsu38e, heme oxygenase (decycling) 1 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | NM_010442.2 |
| 基因名称 | HO-1 |
| Gene ID (NCBI) | 15368 |
| RRID | AB_3670958 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P14901 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HO-1/Hmox1 antibody 83291-3-RR | Download protocol |
| WB protocol for HO-1/Hmox1 antibody 83291-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
