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Host
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Species Reactivity
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0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

    WB analysis using 83291-3-RR (same clone as 83291-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

    IHC staining of mouse spleen using 83291-3-RR (same clone as 83291-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

    FC experiment of NIH/3T3 using 83291-3-RR (same clone as 83291-3-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83291-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83291-3-RR

    Affinity and Kinetic Characterization of 83291-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

Mouse HO-1/Hmox1 Recombinant antibody, PBS Only

HO-1/Hmox1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83291-3-PBS

产品说明书

CloneNo. 240236G4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Hmox1, HO-1, 240236G4, D8Wsu38e, heme oxygenase (decycling) 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83291-3-PBS targets HO-1/Hmox1 in WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 HO-1/Hmox1 fusion protein Eg0938 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heme oxygenase (decycling) 1
别名 Hmox1, HO-1, 240236G4, D8Wsu38e, heme oxygenase (decycling) 1
计算分子量33 kDa
GenBank蛋白编号NM_010442.2
基因名称 HO-1
Gene ID (NCBI) 15368
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14901
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C