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验证数据展示

  • WB analysis of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

    WB analysis of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

    WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • WB analysis using 10701-1-AP (same clone as 10701-1-PBS)

    WB analysis using 10701-1-AP (same clone as 10701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

    IP experiment of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

    IP result of anti-HO-1/HMOX1 (IP:10701-1-AP, 4ug; Detection:10701-1-AP 1:20000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    IHC staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

    FC experiment of HeLa using 10701-1-AP (same clone as 10701-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    IF Staining of mouse spleen using 10701-1-AP (same clone as 10701-1-PBS)

    Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10701-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HO-1/HMOX1 Polyclonal antibody, PBS Only

HO-1/HMOX1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA
Cat No. 10701-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA

别名

HMOX1, HO-1, bK286B10, EC:1.14.14.18, heme oxygenase (decycling) 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.

产品信息

10701-1-PBS targets HO-1/HMOX1 in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1190

Product name: Recombinant human HO-1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-288 aa of BC001491

Sequence: MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQHYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heme oxygenase (decycling) 1
别名 HMOX1, HO-1, bK286B10, EC:1.14.14.18, heme oxygenase (decycling) 1
计算分子量 33 kDa
观测分子量 28-33 kDa
GenBank蛋白编号BC001491
基因名称 HO-1
Gene ID (NCBI) 3162
RRIDAB_2118685
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09601
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

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