• Featured Product
  • KD/KO Validated

HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for WB, IP, IF-P, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IF-P, IHC, ELISA, FC (Intra) and More (1)

Conjugate

Unconjugated

Cat No : 10701-1-AP

Print datasheet

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32



经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, rat spleen tissue, HepG2 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10701-1-AP targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF-P, IHC, ELISA, FC (Intra) Application Description
Cited ApplicationsWB, IHC, IP, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, bovine, goat
Immunogen HO-1/HMOX1 fusion protein Ag1190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heme oxygenase (decycling) 1
Synonyms bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 28-33 kDa
GenBank Accession NumberBC001491
Gene Symbol HO-1
Gene ID (NCBI) 3162
RRIDAB_2118685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09601
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

实验方案

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IHC protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IF protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IP protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
FC protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

MFSD7c functions as a transporter of choline at the blood-brain barrier

Authors - Xuan Thi Anh Nguyen
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
WB

Cell Metab

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Authors - Timothy C Kenny
mouseWB

Cell Metab

Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.

Authors - Xiphias Ge Zhu
mouseIHC

ACS Nano

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Authors - Xirui Xin
humanIHC

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang