- Featured Product
- KD/KO Validated
HO-1 Recombinant antibody
HO-1 Recombinant Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat and More (3)
Applications
IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2M6
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, U2OS cells, HSC-T6 cells, rat spleen tissue |
Positive IP detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 17 publications below |
产品信息
81281-1-RR targets HO-1 in WB, IP, ELISA applications and shows reactivity with Human, Rat samples.
Tested Applications | IP, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | Human, Rat |
Cited Reactivity | human, mouse, rat |
Immunogen | HO-1 fusion protein Ag1190 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | heme oxygenase (decycling) 1 |
Synonyms | bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32 |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC001491 |
Gene Symbol | HMOX1 |
Gene ID (NCBI) | 3162 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P09601 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HO-1 antibody 81281-1-RR | Download protocol |
IP protocol for HO-1 antibody 81281-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Dis Negative regulation of pro-apoptotic AMPK/JNK pathway by itaconate in mice with fulminant liver injury | ||
Ecotoxicol Environ Saf Yttrium chloride induces ferroptosis in cardiomyocytes via iron accumulation and triggers cardiac lipid peroxidation and inflammation that cause heart adverse events in mice | ||
J Agric Food Chem Lactoferrin Relieves Deoxynivalenol-Induced Oxidative Stress and Inflammatory Response by Modulating the Nrf2/MAPK Pathways in the Liver | ||
Int J Mol Sci Red Ginseng Improves D-galactose-Induced Premature Ovarian Failure in Mice Based on Network Pharmacology | ||
Eur J Pharmacol The protective effects of uric acid against myocardial ischemia via the Nrf2 pathway | ||
Eur J Pharmacol Kinsenoside alleviates oxidative stress-induced blood-brain barrier dysfunction via promoting Nrf2/HO-1 pathway in ischemic stroke |