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验证数据展示

  • IF Staining of HepG2 using CL488-12770

    IF Staining of HepG2 using CL488-12770

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HNRNPD antibody (CL488-12770) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-12770

    FC experiment of HepG2 using CL488-12770

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 HNRNPD Polyclonal Antibody (CL488-12770)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated HNRNPD Polyclonal antibody

HNRNPD Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12770

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

AUF1, HNRPD, hnRNPD0, hnRNP D0, AU-rich element RNA-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPD Ready-To-Use IHC Kit. HNRNPD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12770-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12770 targets HNRNPD in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 HNRNPD fusion protein Ag3458 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
别名 AUF1, HNRPD, hnRNPD0, hnRNP D0, AU-rich element RNA-binding protein 1
计算分子量 355 aa, 38 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC026015
基因名称 HNRNPD
Gene ID (NCBI) 3184
RRIDAB_3672548
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ14103
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HNRNPD antibody CL488-12770Download protocol
FC protocol for CL Plus 488 HNRNPD antibody CL488-12770Download protocol
Standard Protocols
Click here to view our Standard Protocols