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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68236-1-Ig (same clone as 68236-1-PBS)

    WB analysis using 68236-1-Ig (same clone as 68236-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68236-1-Ig (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 68236-1-Ig (same clone as 68236-1-PBS)

    IHC staining of human cervical cancer using 68236-1-Ig (same clone as 68236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPD antibody included
  • IHC staining of human cervical cancer using 68236-1-Ig (same clone as 68236-1-PBS)

    IHC staining of human cervical cancer using 68236-1-Ig (same clone as 68236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPD antibody included
  • IF Staining of HepG2 using 68236-1-Ig (same clone as 68236-1-PBS)

    IF Staining of HepG2 using 68236-1-Ig (same clone as 68236-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (68236-1-Ig, Clone: 2G9E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

HNRNPD Monoclonal antibody, PBS Only

HNRNPD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68236-1-PBS

产品说明书

CloneNo. 2G9E6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HNRNPD Ready-To-Use IHC Kit. HNRNPD primary antibody included.

产品信息

68236-1-PBS targets HNRNPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 HNRNPD fusion protein Ag3458 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
别名 AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37
计算分子量 355 aa, 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC026015
基因名称 HNRNPD
Gene ID (NCBI) 3184
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14103
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).