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验证数据展示

  • WB analysis using 68236-1-Ig

    WB analysis using 68236-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68236-1-Ig (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human cervical cancer using 68236-1-Ig

    IHC staining of human cervical cancer using 68236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPD antibody included
  • IHC staining of human cervical cancer using 68236-1-Ig

    IHC staining of human cervical cancer using 68236-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPD antibody included
  • IF Staining of HepG2 using 68236-1-Ig

    IF Staining of HepG2 using 68236-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (68236-1-Ig, Clone: 2G9E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

HNRNPD Monoclonal antibody

HNRNPD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68236-1-Ig

产品说明书

CloneNo. 2G9E6
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, pig, rabbit and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPD Ready-To-Use IHC Kit. HNRNPD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68236-1-Ig targets HNRNPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat, pig, rabbit
文献引用反应性human
免疫原

CatNo: Ag3458

Product name: Recombinant human HNRNPD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC026015

Sequence: MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
别名 AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37
计算分子量 355 aa, 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC026015
基因名称 HNRNPD
Gene ID (NCBI) 3184
RRIDAB_2935323
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14103
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).

实验方案

Product Specific Protocols
WB protocol for HNRNPD antibody 68236-1-IgDownload protocol
IHC protocol for HNRNPD antibody 68236-1-IgDownload protocol
IF protocol for HNRNPD antibody 68236-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Tissue Cell

Activation of the SIRT1/PGC-1α pathway by HNRNPD promotes vasculogenic mimicry in NSCLC

Authors - Xuefeng Zhang
View Article