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验证数据展示

  • WB analysis using 68447-1-Ig (same clone as 68447-1-PBS)

    WB analysis using 68447-1-Ig (same clone as 68447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68447-1-Ig (HNRNPC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

    IP experiment of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

    IP result of anti-HNRNPC (IP:68447-1-Ig, 4ug; Detection:68447-1-Ig 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

    IF Staining of HeLa using 68447-1-Ig (same clone as 68447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (68447-1-Ig, Clone: 2G8G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68447-1-PBS in a different storage buffer formulation.

HNRNPC Monoclonal antibody, PBS Only

HNRNPC Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 68447-1-PBS

产品说明书

CloneNo. 2G8G4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse , rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HNRNPC Ready-To-Use IHC Kit. HNRNPC primary antibody included.

产品信息

68447-1-PBS targets HNRNPC in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse , rat
免疫原

CatNo: Ag2356

Product name: Recombinant human HNRNPC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-293 aa of BC003394

Sequence: MASNVTNKTDPRSMNSRVFIGNLNTLVVKKSDVEAIFSKYGKIVGCSVHKGFAFVQYVNERNARAAVAGEDGRMIAGQVLDINLAAEPKVNRGKAGVKRSAAEMYGSSFDLDYDFQRDYYDRMYSYPARVPPPPPIARAVVPSKRQRVSGNTSRRGKSGFNSKSGQRGSSKSGKLKGDDLQAIKKELTQIKQKVDSLLENLEKIEKEQSKQAVEMKNDKSEEEQSSSSVKKDETNVKMESEGGADDSAEEGDLLDDDDNEDRGDDQLELIKDDEKEAEEGEDDRDSANGEDDS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein C (C1/C2)
别名 C1, C2, HNRNP, hnRNP C1/C2, HNRNPC, HNRPC, SNRPC
计算分子量 32 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC003394
基因名称 HNRNPC
Gene ID (NCBI) 3183
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP07910
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPC, also named as Heterogeneous nuclear ribonucleoproteins C1/C2, is a 306 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and belongs to the RRM HNRPC family. HNRNPC localizes in the nucleus. It binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. HNRNPC may play a role in the early steps of spliceosome assembly and pre-mRNA splicing. HNRNPC can act as a tetramer and is involved in the assembly of 40S hnRNP particles and plays a role with CSDE1 in internal initiation of translation during mitosis.

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