验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67445-1-Ig targets HNRNPA2B1 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag6699 Product name: Recombinant human HNRNPA2B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-249 aa of BC000506 Sequence: MEREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEDLEVAILEVAPVMEEEEEDMVVEDLDMATRVGATEVVMTTMEEEIMEVEITMILEIITSNLLTTVQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein A2/B1 |
| 别名 | DKFZp779B0244, FLJ22720, hnRNP A2/B1, HNRNPA2, HNRNPA2B1, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC000506 |
| 基因名称 | HNRNPA2B1 |
| Gene ID (NCBI) | 3181 |
| RRID | AB_2882679 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22626 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HnRNPA2B1 is one of heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA and participate in carcinogenesis. It has a role in packaging nascent mRNA, alternative splicing, tycoplamic RNA trafficking, translation and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPA2B1 antibody 67445-1-Ig | Download protocol |
| IHC protocol for HNRNPA2B1 antibody 67445-1-Ig | Download protocol |
| WB protocol for HNRNPA2B1 antibody 67445-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med HNRNPA2B1-mediated m6A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis
| ||
Cancer Lett HNRNPA2B1 regulates tamoxifen- and fulvestrant-sensitivity and hallmarks of endocrine resistance in breast cancer cells. | ||
Stem Cell Res Ther Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.
| ||
J Virol VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1 | ||
Clin Transl Med Exosome-derived long non-coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β-catenin signalling | ||
J Nanobiotechnology Exosome-delivered NR2F1-AS1 and NR2F1 drive phenotypic transition from dormancy to proliferation in treatment-resistant prostate cancer via stabilizing hormonal receptors |
