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验证数据展示

  • WB analysis using 34183-1-AP (same clone as 34183-1-PBS)

    WB analysis using 34183-1-AP (same clone as 34183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 34183-1-AP (HMX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 34183-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 34183-1-AP (same clone as 34183-1-PBS)

    IHC staining of mouse liver using 34183-1-AP (same clone as 34183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 34183-1-AP (HMX3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34183-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 34183-1-AP (same clone as 34183-1-PBS)

    IF Staining of HeLa using 34183-1-AP (same clone as 34183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMX3 antibody (34183-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 34183-1-PBS in a different storage buffer formulation.

HMX3 Polyclonal antibody, PBS Only

HMX3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 34183-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Homeobox protein H6 family member 3, Homeobox protein HMX3, Homeobox protein Nkx-5.1, NKX-5.1, NKX5-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

34183-1-PBS targets HMX3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag41398

Product name: Recombinant human HMX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-169 aa of NM_001105574

Sequence: GFALSQVGDLAFPRFEIPAQRFALPAHYLERSPAWWYPYTLTPAGGHLPRPEASEKALLRDSSPASGTDRDSPEPLLKADPDHKELDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 H6 family homeobox 3
别名 Homeobox protein H6 family member 3, Homeobox protein HMX3, Homeobox protein Nkx-5.1, NKX-5.1, NKX5-1
计算分子量357aa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号NM_001105574
基因名称 HMX3
Gene ID (NCBI) 340784
RRIDAB_3743167
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDA6NHT5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

H6 family homeobox 3 (HMX3) is also named Homeobox protein H6 family member 3 and NKX-5.1. HMX3 is identified as a protein-coding gene, and diseases associated with HMX3 primarily include upper semicircular canal dehiscence and semicircular canal dehiscence syndrome (PMID: 22779713). In addition, as a transcription factor, HMX3 is involved in the regulation of nerve cell types, which is essential for the development of the inner ear and hypothalamus (PMID: 27151948, PMID: 15363417). HMX3 is lowly expressed in CRC, and HMX3 expression was negatively correlated with poor prognosis of CRC patients (PMID: 36204976).

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