验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34183-1-AP targets HMX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag41398 Product name: Recombinant human HMX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 82-169 aa of NM_001105574 Sequence: GFALSQVGDLAFPRFEIPAQRFALPAHYLERSPAWWYPYTLTPAGGHLPRPEASEKALLRDSSPASGTDRDSPEPLLKADPDHKELDS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | H6 family homeobox 3 |
| 别名 | Homeobox protein H6 family member 3, Homeobox protein HMX3, Homeobox protein Nkx-5.1, NKX-5.1, NKX5-1 |
| 计算分子量 | 357aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | NM_001105574 |
| 基因名称 | HMX3 |
| Gene ID (NCBI) | 340784 |
| RRID | AB_3743167 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | A6NHT5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
H6 family homeobox 3 (HMX3) is also named Homeobox protein H6 family member 3 and NKX-5.1. HMX3 is identified as a protein-coding gene, and diseases associated with HMX3 primarily include upper semicircular canal dehiscence and semicircular canal dehiscence syndrome (PMID: 22779713). In addition, as a transcription factor, HMX3 is involved in the regulation of nerve cell types, which is essential for the development of the inner ear and hypothalamus (PMID: 27151948, PMID: 15363417). HMX3 is lowly expressed in CRC, and HMX3 expression was negatively correlated with poor prognosis of CRC patients (PMID: 36204976).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HMX3 antibody 34183-1-AP | Download protocol |
| IHC protocol for HMX3 antibody 34183-1-AP | Download protocol |
| WB protocol for HMX3 antibody 34183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
