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验证数据展示

  • WB analysis of SKOV-3 using 17206-1-AP

    WB analysis of SKOV-3 using 17206-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17206-1-AP (HMGCLL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17206-1-AP

    IHC staining of human liver cancer using 17206-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17206-1-AP (HMGCLL1 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver cancer using 17206-1-AP

    IHC staining of human liver cancer using 17206-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17206-1-AP (HMGCLL1 antibody) at dilution of 1:200 (under 10x lens).

HMGCLL1 Polyclonal antibody

HMGCLL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17206-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HMGCLL1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17206-1-AP targets HMGCLL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10985

Product name: Recombinant human HMGCLL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-340 aa of BC024194

Sequence: MGNVPSAVKHCLSYQQLLREHLWIGDSVAGALDPAQETSQLSGLPEFVKIVEVGPRDGLQNEKVIVPTDIKIEFINRLSQTGLSVIEVTSFVSSRWVPQMADHTEVMKGIHQYPGVRYPVLTPNLQGFHHAVAAGATEISVFGAASESFSKKNINCSIEESMGKFEEVVKSARHMNIPARGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKRMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGLGLNTGVNLYKVMEAGDFICKAVNKTTNSKVAQASFNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1
别名 HMGCLL1
计算分子量 340 aa, 36 kDa
观测分子量 32 kDa
GenBank蛋白编号BC024194
基因名称 HMGCLL1
Gene ID (NCBI) 54511
RRIDAB_2878362
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TB92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGCLL1 (3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1), also named as er-cHL, is a close homolog of mitochondrial HMG-CoA lyase (mHL). HMGCLL1 catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, which is a key step in ketogenesis (PMID: 22847177, 22865860). HMGCLL1 has some isoforms with the molecular mass of 32-39 kDa and 6 kDa.

实验方案

Product Specific Protocols
IHC protocol for HMGCLL1 antibody 17206-1-APDownload protocol
WB protocol for HMGCLL1 antibody 17206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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