验证数据展示
经过测试的应用
Positive WB detected in | SKOV-3 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17206-1-AP targets HMGCLL1 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | HMGCLL1 fusion protein Ag10985 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1 |
别名 | HMGCLL1 |
计算分子量 | 340 aa, 36 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC024194 |
基因名称 | HMGCLL1 |
Gene ID (NCBI) | 54511 |
RRID | AB_2878362 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TB92 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HMGCLL1 (3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1), also named as er-cHL, is a close homolog of mitochondrial HMG-CoA lyase (mHL). HMGCLL1 catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, which is a key step in ketogenesis (PMID: 22847177, 22865860). HMGCLL1 has some isoforms with the molecular mass of 32-39 kDa and 6 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for HMGCLL1 antibody 17206-1-AP | Download protocol |
IHC protocol for HMGCLL1 antibody 17206-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |