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验证数据展示

  • WB analysis using 84769-4-RR

    WB analysis using 84769-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84769-4-RR

    FC experiment of HeLa using 84769-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84769-4-RR

    Affinity and Kinetic Characterization of 84769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

HMGCL Recombinant monoclonal antibody

HMGCL Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84769-4-RR

产品说明书

CloneNo. 242224F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

242224F9, EC:4.1.3.4, HL, HMG CoA lyase, HMG-CoA lyase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84769-4-RR targets HMGCL in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10426

Product name: Recombinant human HMGCL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-325 aa of BC010570

Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
别名 242224F9, EC:4.1.3.4, HL, HMG CoA lyase, HMG-CoA lyase
计算分子量 325 aa, 34 kDa
观测分子量 32 kDa
GenBank蛋白编号BC010570
基因名称 HMGCL
Gene ID (NCBI) 3155
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP35914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

实验方案

Product Specific Protocols
FC protocol for HMGCL antibody 84769-4-RRDownload protocol
WB protocol for HMGCL antibody 84769-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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