验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human liver tissue, human ovary tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16898-1-AP targets HMGCL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10426 Product name: Recombinant human HMGCL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC010570 Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase |
| 别名 | HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4 |
| 计算分子量 | 325 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC010570 |
| 基因名称 | HMGCL |
| Gene ID (NCBI) | 3155 |
| RRID | AB_2295304 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35914 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HMGCL antibody 16898-1-AP | Download protocol |
| IP protocol for HMGCL antibody 16898-1-AP | Download protocol |
| WB protocol for HMGCL antibody 16898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma. | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Hepatol Int HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility | ||
J Biol Chem Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation | ||
Int J Biol Sci Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer
| ||
Cell Rep Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria |
