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验证数据展示

  • WB analysis of mouse testis using 12787-1-AP (same clone as 12787-1-PBS)

    WB analysis of mouse testis using 12787-1-AP (same clone as 12787-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12787-1-AP (HMGB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12787-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 12787-1-AP (same clone as 12787-1-PBS)

    WB analysis of human testis using 12787-1-AP (same clone as 12787-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 12787-1-AP (HMGB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12787-1-PBS in a different storage buffer formulation.

HMGB4 Polyclonal antibody, PBS Only

HMGB4 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12787-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12787-1-PBS targets HMGB4 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3503

Product name: Recombinant human HMGB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC021180

Sequence: MGKEIQLKPKANVSSYVHFLLNYRNKFKEQQPSTYVGFKEFSRKCSEKWRSISKHEKAKYEALAKLDKARYQEEMMNYVGKRKKRRKRDPQAPRRPPSSFLLFCQDHYAQLKRENPNWSVVQVAKATGKMWSTATDLEKHPYEQRVALLRAKYFEELELYRKQCNARKKYRMSARNRCRGKRVRQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 high-mobility group box 4
别名
计算分子量 186 aa, 22 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC021180
基因名称 HMGB4
Gene ID (NCBI) 127540
RRIDAB_10638490
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WW32
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

High mobility group protein B4 (HMGB4) is a new member in the family of HMGB proteins. Unlike other HMGB-proteins, it is a mammalian specific protein that has two HMGB-boxes but lacks the acidic tail. HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues (PMID: 18987332). It binds strongly to nuclear structures, regulates chromatin and mediates differentiation of neuronal cells (PMID: 27608812).

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